钠碘转运体、甲状腺过氧化物酶、Ⅰ型脱碘酶基因在桥本甲状腺炎合并乳头状癌患者中的作用

目的 观察甲状腺钠碘转运体(NIS)、过氧化物酶(TPO)、Ⅰ型脱碘酶(DIOI)基因突变在桥本甲状腺炎(HT)合并乳头状癌(PTC)患者发病中的作用.方法 选择34例HT合并PTC的患者作为病例组和26例HT患者作对照组,在病理切片指导下获取HT和PTC组织标本并提取其基因组DNA,对目的基因片段进行聚合酶链反应(PCR)及DNA测序.结果 未见NIS基因变化,发现6种TPO基因点突变和1种碱基插入性突变,同时可见3种DIOI基因点突变,两组突变率差异有统计学意义(P＜0.05),同时在病例组不同的促甲状腺激素(TSH)水平突变率不同(P＜0.05).结论 TPO、DIOI等基因突变在HT合并PTC的形成中发挥显著作用.     

A simple approach for enhanced immune response using engineered dendritic cell targeted nanoparticles

In this study, we demonstrate a simple strategy for enhanced immune response using a two-component dendritic cell (DC) targeted antigen delivery system. One component consists of a recombinant bifunctional fusion protein (bfFp) used for DC targeting, whereas, the other component is made of biotinylated PLGA nanoparticles that encapsulate the antigen. The fusion protein (bfFp) made of a truncated core-streptavidin fused to anti-DEC-205 single chain antibody (scFv) was mixed with ovalbumin-loaded biotinylated NPs that were formulated using biotin–PEG (2000)–PLGA, and the combination, bfFp functionalized NPs was used for DC targeted antigen delivery. In vitro DC uptake studies revealed a 2-fold higher receptor-mediated uptake of bfFp functionalized NPs when compared to non-targeted NPs. Immunization of the mice with the bfFp functionalized NPs in conjunction with DC maturation stimulus (anti-CD40 mAb) enhanced OVA-specific IgG and IgG subclass responses. Splenocytes of these mice secreted significantly higher levels of Th1 (IFN-γ and IL-2) cytokines upon ex vivo restimulation with OVA. The promising outcomes of the bfFp functionalized DC targeted system support its use as a versatile vaccine delivery system for the design of monovalent or polyvalent vaccines.     

亚硝酸钠对心肌谷胱甘肽过氧化物酶的影响及维生素E和硒的保护作用

大白鼠喂饲克山病病区粮,并分别向病区粮加维生素 E 和硒,观察在亚硝酸钠急性中毒情况下各组大鼠心肌谷胱甘肽过氧化物酶的变化。结果表明,亚硝酸钠可使病区粮所饲大鼠心肌谷胱甘肽过氧化物酶活性明显下降,补充维生素 E 或硒则可保护此酶活性防止其下降。提出除低硒外,亚硝酸盐过多及维生素 E 缺乏可能参与了克山病发病环节。     

阿托伐他汀对急性心肌梗死髓过氧化物酶的影响

目的探讨阿托伐他汀对急性心肌梗死髓过氧化物酶的影响。方法雄性SD大鼠结扎左冠状动脉前降支建立心肌梗死模型,并随机分为治疗组(19只)、对照组(19只)和假手术组(18只),治疗组术前给予阿托伐他汀10mg.kg-1.d-1,对照组和假手术组给予相应体积的生理盐水。瑞吉染色法检测血液中中性粒细胞(polymorph nuclear neutrophil,PMN)的数量;HE染色光镜检测心肌组织中PMN浸润的数量;TTC染色法测定心肌梗死范围;髓过氧化物酶(MPO)法测定血清和心肌MPO活性。结果治疗组和对照组血液、心肌内的PMN浸润较假手术组明显增加(P<0.01),但治疗组较对照组PMN数量明显减少(P<0.05)。治疗组和对照组血清、心肌内MPO活性均较假手术组明显增加(P<0.01);与对照组比较,治疗组血清、心肌内MPO活性明显降低(P<0.05)。治疗组和对照组心肌梗死范围与假手术组比较有显著差异(P<0.01);治疗组与对照组比较,心肌梗死范围明显缩小(P<0.05)。结论急性心肌梗死后PMN浸润,MPO活性增加,阿托伐他汀干预后可抑制PMN浸润和MPO活性,减少心肌梗死面积。     

谷光甘肽、丙二醛联合检测对肺癌的诊断价值

目的　分析肺癌患者血清中谷光甘肽 (GSH PX)、丙二醛 (MDA)的水平及其相关性 ,探讨其作为肺癌筛选指标的可能性。方法　用DTNB法对 5 2例肺癌、34例非肿瘤性肺病患者 (配比组 )及 30名健康人对照组血清中GSH PX活力进行测定 ,用TAB法测定其MDA的含量。结果　血清中GSH PX活力呈现肺癌组 [( 110 .80± 31.78)活力单位 ]小于配比组 [( 132 .30± 37.35 )活力单位 ]小于对照组 [( 16 4.2 4± 33 .49)活力单位 ]的梯度 (P <0 .0 5 ) ;肺癌组MDA [( 8.2 2± 3 .91)nmol/ml]明显高于配比组 [( 5 .6 9± 2 .38)nmol/ml]和对照组 [( 4 .36± 1.42 )nmol/ml] ,两者比较差异具有显著性 (P <0 .0 5 ) ,配比组虽然高于对照组 ,但差异无显著性 ;肺癌组中GSH PX与MDA呈明显的负相关 (r =-0 .15 2 0 ,P <0 .0 5 )。结论　联合检测GSH PX、MDA的变化对高危人群的健康监护及其肺癌的筛检可能具有一定的意义。     

The effect of bilirubin on biliary lipid secretion: Analysis by horseradish peroxidase associated intrahepatic vesicular transport system

The mechanism of biliary lipid secretion is still controverial and there is no definite information regarding how bilirubin inhibits biliary phospholipid and cholesterol secretions without affecting bile salt secretion. In this study, the effects of biliribin on intrahepatic vesicular transport and biliary lipid secretion were examined using bile-fistula rats. Horseradish peroxidase (HRP) was used as a tracer of intrahepatic vesicular transport. Bilirubin (5mg/100g BW) and/or HRP (5mg/100gBW) were injected through the mesenteric vein. Bile flow, biliary bile acid, biliary phospholipid and cholesterol outputs were examined in saline, HRP and HRP+bilirubin groups, respectively. Bile flow and biliary bile acid output were not affected by bilirubin administration. Biliary phospholipid and cholesterol as well as biliary HRP outputs were inhibited just after bilirubin administration, 42.8±6.1 SD% 47.7±5.1 SD%, and 33.4±3.8 SD%, respectively. These results suggested the participation of intrahepatic vesicular transport system in the inhibition of biliary lipid secretion by bilirubin and in its secretory mechanism. This study was partially funded by Uehara Life Science Foundation.     

谷胱甘肽过氧化物酶1基因多态性与川崎病的关联性分析

目的探讨谷胱甘肽过氧化物酶1(GPX-1)基因多态性与中国汉族儿童川崎病(kawasaki disease,KD)并冠状动脉损害(CAL)的相关性。方法采用聚合酶链反应-限制性内切酶片段长度多态性分析(PCR-RFLP)检测92例川崎病患儿及108例健康儿童GPX-1基因的-46C/T位点和599C/T位点多态性。结果川崎病组GPX-1基因-46C/T位点CC、CT、TT基因型分布和C、T等位基因频率与正常对照组比较差异无显著性意义(χ2=0.174和0.166,P均0.05)。川崎病组GPX-1基因599C/T位点CC、CT、TT基因型分布和C、T等位基因频率与正常对照组比较差异亦无显著性意义(χ2=0.429和0.368,P均0.05)。川崎病患儿合并CAL组与无冠状动脉损害(NCAL)组GPX-1基因-46C/T位点基因型分布和等位基因频率比较差异亦无显著性意义(χ2=0.507和0.487,P均0.05)。川崎病患儿合并CAL组与NCAL组GPX-1基因599C/T位点基因型分布和等位基因频率比较差异亦无显著性意义(χ2=0.635和0.535,P均0.05)。结论尚未发现GPX-1基因-46C/T位点多态性以及599C/T位点多态性与川崎病及其CAL的发生存在明显关联性。     

Radiation sensitivities in various anticancer-drug-resistant human lung cancer cell lines and mechanism of radiation cross-resistance in a cisplatin-resistant cell line

Summary To determine whether there exists cross-resistance between anticancer drugs and radiation, six drug-resistant human lung cancer cell lines and their parental cell lines were examined for radiosensitivity using a growth-inhibition assay. Only one cisplatin-resistant cell line, PC-9/CDDP, showed cross-resistance to radiation. The other three cisplatin-resistant cell lines (PC-7/CDDP, PC-4/CDDP, and H69/CDDP), an etoposide-resistant cell line (H69/VP) and a camptothecin-resistant cell line (PC-7/CPT) did not show cross-resistance to radiation. To analyze the mechanism of radiation resistance in PC-9/CDDP cells, the formation and repair of radiation-induced DNA single-strand breaks (ssb) and double-strand breaks (dsb) were examined by alkaline elution and neutral elution respectively. Although the formation of DNA ssb and repair of both DNA ssb and DNA dsb were the same for both cell lines, the formation of DNA dsb in PC-9/CDDP cells was significantly less than those in PC-9 cells. Measurement of intracellular glutathione content in all of the cell lines revealed that only PC-9/CDDP cells had a significant increase of glutathione content compared to the parental cells. Buthionine sulfoximine treatment of PC-9/CDDP cells caused an increase of DNA dsb to the same levels as in PC-9 cells after irradiation and caused a complete radiosensitization. These results indicate that cross-resistance to radiation in drug-resistant cells in a rare phenomenon, and increased glutathione content may play a crucial role in the emergence of cross-resistance to radiation in the drug-resistant cells.Abbreviations Cisplatin cis-diamminedichloroplatinum(II) - VP-16 etoposide - GSH glutathione - FBS fetal bovine serum - BSO L-buthionine (S, R)-sulfoximine - ssb single-strand breaks - dsb doublestrand breaks     

The interrelation of glutathione reductase,catalase, glutathione peroxidase,superoxide dismutase,and glucose-6-phosphate in the pathogenesis of rheumatoid arthritis

Rheumatoid arthritis (RA) is the most common form of inflammatory arthritis, a systemic autoimmune disease characterized by chronic inflammation of the synovial joints, ultimately leading to joint destruction and permanent disability, affecting 1% of the world population. Oxidative stress in rheumatoid inflammation, due to the fact that antioxidant systems are impaired in RA and caused by fee radicals, might have an essential role in etiology of RA. This review includes the interrelation of antioxidants against free radicals in RA patients. There is much evidence that antioxidant team that covers glutathione reductase, catalase, glutathione peroxidase, superoxide dismutase, and glucose-6-phopshate destroy reactive oxygen species and other free radicals through enzymatic as well as nonenzymatic means. The change in relative levels of antioxidants vis-à-vis free radical formation and level could be used as indicators for effective and earlier diagnosis of RA.